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MCF-7 Cas9-expressing cells were transduced in independent triplicate samples of 1.73 10 8 cells each with the lentiviral whole-genome sgRNA library at a multiplicity of infection of 0.25-0.29, as measured by puromycin resistance, to achieve a representation of 252287 cells per sgRNA

Whereas phenotypic heterogeneity can be viewed as a problem in current bioproduction processes, as described above, it may also present an opportunity to improve yield via potential enrichment of high-producing cells at the expense of low-producing cells

Over weeks, this accumulates into meaningful dose deviation

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Altman DG, Andersen PK